A Strategy to Setup Codominant Microsatellite Analysis for High-Resolution-Melting-Curve-Analysis (HRM)
نویسندگان
چکیده
منابع مشابه
Detection of Microsatellite Instability by High-Resolution Melting Analysis in Colorectal Cancer
Background: Colorectal cancer (CRC) is the third most common cancer worldwide. microsatellite instability (MSI) is a molecular marker of a deficient mismatch repair system and happens in almost 15% of CRCs. Because of a wide frequency of MSI+ CRC in Iran compared to other parts of the world, the importance of screening for this type of cancer is highlighted. Methods: The most common MSI detecti...
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روش های متنوعی برای شناسایی جهش ها ابداع شده است که هریک از آنها دارای مزایا و معایب خاص خود هستند. یکی از روش هایی که اخیراً ابداع شده و در مطالعات مختلف به کارآیی بالای آن اشاره شده است روش high resolution melting analysis یا hrm است. در مطالعه حاضر برای اولین بار در ایران به منظور شناسایی جهش ها تمامی اگزون های ژن rb1 با استفاده از روش تعیین توالی بررسی شده است. در ادامه به منظور بررسی حساسیت...
Comparison of cattle BoLA-DRB3 typing by PCR-RFLP, direct sequencing, and high-resolution DNA melting curve analysis
Major histocompatibility complex (MHC) represents an important genetic marker for manipulation to improve the health and productivity of cattle. It is closely associated with numerous disease susceptibilities and immune responses. Bovine MHC, also called bovine leukocyte antigen (BoLA), is considered as a suitable marker for genetic diversity studies. In cattle, most of the polymorphisms are lo...
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ژورنال
عنوان ژورنال: BMC Genetics
سال: 2008
ISSN: 1471-2156
DOI: 10.1186/1471-2156-9-69